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目的:制备白藜芦醇固体脂质纳米粒(Res-SLN),研究其理化特性及体外抗肿瘤作用。方法:采用溶剂乳化挥发法制备Res-SLN,并用透射电镜观察纳米粒的形态;激光粒度分析仪检测粒径分布和Zeta电位;高效液相色谱法测定载药量、包封率及体外释药;MTT法评价体外抗肿瘤作用。结果:制备的Res-SLN为类球形,粒径分布均匀,平均粒径为96.7nm,zata电位为-16.3mv,载药量和包封率分别为(7.95±0.21)%和(91.34±0.18)%,体外释放缓慢,对HepG2细胞具有较强的抑制作用。结论:制备的Res-SLN包封率和载药量高,粒径均匀,体外释放具有缓释特点,体外抗肿瘤作用强。
Objective: To prepare resveratrol solid lipid nanoparticles (Res-SLN), to study its physical and chemical properties and anti-tumor effect in vitro. Methods: Res-SLN was prepared by solvent emulsification and volatilization. The morphology of nanoparticles was observed by transmission electron microscopy. The particle size distribution and Zeta potential were measured by laser particle size analyzer. The drug loading, entrapment efficiency and drug release in vitro were determined by high performance liquid chromatography ; MTT evaluation of anti-tumor effect in vitro. Results: The prepared res-SLN was spheroidal and had a uniform particle size distribution with a mean particle size of 96.7 nm and zata potential of -16.3 mV. The drug loading and encapsulation efficiency were (7.95 ± 0.21)% and (91.34 ± 0.18 )%, Slow release in vitro, has a strong inhibitory effect on HepG2 cells. Conclusion: The prepared Res-SLN has high entrapment efficiency and drug loading, uniform particle size, sustained release in vitro and strong antitumor activity in vitro.