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本研究以桑白皮药渣为研究对象,对其酶解工艺进行了优化,探究中药药渣作为生产高附加值化学品及生物燃料的潜能。结果显示,桑白皮药渣酸处理前后纤维素含量分别为52.5%,47%,含量较高,表明桑白皮药渣有作为生产高附加值化学品及生物燃料的潜能;选取不同酶用量酶解桑白皮药渣,通过比较产糖量以及酶解率,得到最优酶用量为40 FPU·(g DCW)-1,此条件下酶解后,桑白皮药渣单批酶解中葡萄糖,木糖,阿拉伯糖浓度达到23.82 g·L-1,4.84 g·L-1,3.6 g·L-1,酶解率为45.33%,是未进行酸处理药渣的2.3倍;最后通过分批补料酶解策略,进一步提高桑白皮药渣的产糖量,最终葡萄糖浓度达到38 g·L-1,酶解率为36.19%。从本研究结果来看,桑白皮药渣中纤维素和半纤维素含量高,具有作为生产高附加值化学品及生物燃料的潜能,通过酶解的方式,将其转化为微生物可利用的单糖,通过发酵将其转化为高附加值的化学品、生物燃料等,解决药渣污染等问题,从而实现中药制药生产过程的可持续化,绿色化。
In this research, we took the mulberry white dregs as the research object, optimized its enzymatic hydrolysis process, and explored the potential of traditional Chinese medicine dregs as a high value-added chemical and bio-fuel. The results showed that the content of cellulose before and after the treatment of daphnia magna was 52.5% and 47%, respectively, indicating that mulberry bark dregs had the potential to produce high value-added chemicals and biofuels. The optimal dosage of enzyme was 40 FPU · (g DCW) -1 by enzymatic hydrolysis of mulberry white dregs. After enzymolysis under this condition, The concentration of glucose, xylose and arabinose reached 23.82 g · L-1, 4.84 g · L-1 and 3.6 g · L-1, the rate of hydrolysis was 45.33%, which was 2.3 times of that of acid-free dregs. Through the fed-batch enzymolysis strategy, the sugar content of mulberry white dregs was further increased, the final glucose concentration reached 38 g · L-1, and the rate of hydrolysis was 36.19%. From the results of this study, the content of cellulose and hemicellulose in mulberry white skin dregs is high and has the potential of producing high value-added chemicals and biofuels. By enzymolysis, it can be transformed into microbial available Monosaccharide by fermentation to convert it into high value-added chemicals, biofuels and so on, to solve the problem of dregs pollution, in order to achieve the sustainable and green production process of traditional Chinese medicine.