nanobody相关论文
Development of a nanobody-based immunoassay for the sensitive detection of fibrinogen-like protein 1
Immune checkpoint inhibition is an important strategy in cancer therapy.Blockade of CTLA-4 and PD-1/PD-L1 is well develo......
As drug carriers,polymersomes (Ps) have many advantages over the classical liposomes,including adequate stability,long c......
Specificity Evaluation and Disease Monitoring in Arthritis Imaging with Complement Receptor of the I
Single-photon emission computed tomography combined with micro-CT(SPECT/μCT)imaging using Nanobodies against complement......
Listeria monocytogenes causes Listeriosis,a potentially fatal food borne disease....
Human epidermal growth factor receptor(EGFR)is an effective target for the treatment of many epithelial cancers....
Amyloid fibril assembly is associated with many human disorders,[1] and to approach an inhibitor of amyloid formation th......
Cisplatin and related platinum analogs have been widely employed in the clinic as first-line chemo-drugs for the treatme......
Site-Specific C-Terminal Dinitrophenylation as a Novel Strategy to Reconstitute the Fc Functions for
Nanobody is a class of camelid-derived single-domain antibody that has many potential advantages over conventional antib......
The investigation on protein-like specific functions of nano-particles(NPs) has been a huge challenge.Here,the biocompat......
Immunosensing methods are biosensing techniques based on specific recognition of an antigen-antibody immunocomplex,which......
Development and Application of an Immunoassay for Detection of Cd Based on Specific Nano-body Agains
In this study,p-SCN-Bn-DTPA was chosen as a bifunctional chelator to facilitate the synthesis of antigens for Cd,and......
A highly sensitive fluorescence enzyme immunoassay based on alpaca nanobody-alkaline phosphatase fus
With the rapid development of antibody engineering,single chain fragment of variable(scFv)-alkaline phosphatase(AP)f......
将抗日本血吸虫SEA纳米抗体固定于镀金的石墨烯薄膜上,制备一种新型的阻抗型免疫传感器检测日本血吸虫循环抗原.使用扫描电镜、原......
,A camel anti-lysozyme CDR3 only domain antibody selected from phage display VHH library acts as pot
Mimetics of antibody-binding sites represent particularly interesting targets,however they are difficult to identify.In ......
为防控猪流行性腹泻病毒(PEDV)病的流行,构建了靶向PEDV NB3-NB6-Fc纳米抗体融合蛋白的真核表达载体,并探究能否在毕赤酵母中分泌......
弓形虫表面抗原1(Surface antigen 1,SAG1)是虫体入侵宿主细胞的重要蛋白,与弓形虫的毒力密切相关,属于弓形虫病诊断和疫苗研究的......
抗体是一类能特异性识别并结合抗原的免疫球蛋白,也是机体体液免疫的主要成分。随着科学技术的发展及对抗体研究的深入,抗体的应用......
【目的】构建H9N2禽流感病毒(AIV)纳米抗体(Nbs)酵母双杂交文库,为后期筛选H9N2 AIV Nbs奠定基础。【方法】以H9亚型禽流感疫苗(F......
优化噬菌体展示文库构建策略,获得高品质羊驼源天然纳米抗体T7噬菌体展示文库,并对文库质量进行鉴定。分离羊驼外周血淋巴细胞,提......
本研究旨在获得高效特异性的牛病毒性腹泻病毒(BVDV)NS3(P80)非结构蛋白的纳米抗体。用BVDV灭活疫苗免疫羊驼,测得抗体效价后分离......
利用制备的日本血吸虫虫卵抗原（SEA），免疫健康双峰驼Bactrian camel，分离其外周血淋巴细胞提取总RNA，利用RT—PCR扩增骆驼重链抗体IgG可......
利用霍乱毒素B亚基（Bsubunit of Cholera toxin，CTB）能自组装形成五元环状结构，将霍乱毒素B亚基与纳米抗体（VHH28）进行融合从而达到纳米......
将抗日本血吸虫SEA纳米抗体固定于镀金的石墨烯薄膜上,制备一种新型的阻抗型免疫传感器检测日本血吸虫循环抗原.使用扫描电镜、原......
为建立基于纳米抗体的检测日本血吸虫感染的胶体金免疫层析技术，将已经通过噬菌体文库筛选得到的两株纳米抗体的质粒，在枯草芽孢杆菌......
【目的】为预防和降低黄曲霉毒素污染,在前期探明黄曲霉毒素产毒菌株鉴别标识性分子PO8蛋白的基础上,欲研究建立高灵敏、大容量反......
利用噬菌体展示技术进行纳米抗体库的构建和筛选，获得抗癌胚抗原(CEACAM5)的高亲和力、强特异性纳米抗体，为相关癌症诊断及靶向治疗......
通过对不同系统表达的肿瘤标记物癌胚抗原（CEA）纳米抗体与人Fc融合蛋白（11C12-Fc）的表达、纯化及抗体性质等的比较分析,比较不同表达系......
纳米抗体是一类在生物医药中具有重要应用前景的蛋白药物,然而由于其分子过小极易被清除.为了获得更好的体内代谢,我们通过CD47纳......
目的获得靶向人CD147的纳米抗体。方法以胞外区全长CD147蛋白为靶标,从噬菌体展示文库中筛选CD147的纳米抗体,将筛选获得的单域抗......
It is well established that all camelids have unique antibodies circulating in their blood.Unlike antibodies from all ot......
采用易错聚合酶链式反应技术,对来源于天然纳米抗体文库的抗脱氧雪腐镰刀菌烯醇（deoxynivalenol,DON）纳米抗体进行随机突变,通过优化......
通过分选酶和谷氨酰胺转氨酶催化反应制备了一种由纳米抗体和绿色荧光蛋白sfGFP组成的靶向成像探针.通过设计一类具有两种酶识别结......
肌肉生长抑制素（myostatin，MSTN），又称GDF-8（growth differentiationfactor8），是体内广泛存在的一种糖蛋白，属于转化生长β（transforming gr......
