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目的探讨骨形态发生蛋白9(Bone morphogenetic proteins 9,BMP9)对食管鳞状细胞癌细胞增殖、克隆、迁移、侵袭及细胞周期的影响。方法用AdGFP和AdBMP9分别感染3株食管鳞状细胞癌细胞株ECA109、KYSE150和KYSE180,并设不感染病毒的3种细胞作为对照,采用RT-PCR法检测AdBMP9感染的3株细胞中BMP9基因mRNA的转录水平,MTT法检测病毒感染细胞0~96 h各组细胞的增殖活力,平板克隆法检测各组细胞的克隆形成能力,划痕试验检测各组细胞的迁移能力,Transwell小室试验检测各组细胞的侵袭能力,并对3组ECA109细胞进行细胞周期的检测。结果 AdBMP9感染的3株细胞中BMP9基因mRNA的转录水平均明显提高。与AdGFP感染和未感染病毒的细胞相比,3种细胞在过表达BMP9后,增殖活力明显降低(P<0.05);细胞克隆形成能力均下降(下降率达70%以上),且克隆细胞团明显变小;细胞迁移和侵袭能力均下降;AdBMP9感染的ECA109细胞处于G1期的细胞比例明显上升,S期比例明显下降(P<0.05)。结论 BMP9可明显抑制食管鳞癌细胞的增殖、克隆、迁移和侵袭能力,并将细胞周期阻滞于G1期。
Objective To investigate the effect of bone morphogenetic protein 9 (BMP9) on the proliferation, cloning, migration, invasion and cell cycle of esophageal squamous cell carcinoma. Methods Three esophageal squamous cell carcinoma cell lines, ECA109, KYSE150 and KYSE180, were infected with AdGFP and AdBMP9 respectively. Three kinds of cells without virus infection were used as controls. The expression of BMP9 mRNA . The viability of cells in each group was detected by MTT assay at 0-96 h. The clonogenic capacity of each group of cells was detected by plate clone assay. The migration ability of cells in each group was detected by scratch assay. The Transwell chamber assay was used to detect the cell viability. Cell invasion ability, and three groups of ECA109 cells for cell cycle detection. Results The transcriptional level of BMP9 mRNA in all three AdBMP9-infected cells was significantly increased. Compared with AdGFP-infected and non-infected cells, the proliferation of three kinds of cells was significantly decreased (P <0.05) after BMP9 overexpression; the ability of cell clonality decreased (the descending rate was over 70%), (P <0.05). The percentage of cells in G1 phase in AdBMP9-infected ECA109 cells was significantly increased and the proportion of S phase was significantly decreased (P <0.05). Conclusion BMP9 can significantly inhibit the proliferation, cloning, migration and invasion of esophageal squamous cell carcinoma cells and block the cell cycle in G1 phase.