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目的:建立测定补肾益脑片中梓醇含量的方法。方法:采用高效液相色谱法测定,Amethyst C_(18)-H色谱柱(4.6 mm×150mm,5μm),柱温为室温,流动相为乙腈-0.1%磷酸水溶液(3∶97),流速为0.8 m L/min,检测波长为205 nm。结果:补肾益脑片中的梓醇在2~100μg/m L线性范围内与峰面积线性关系良好,相关系数为0.999 6,平均回收率为95.8%(n=5),RSD为2.15%。结论:本方法简便、快速,可用于补肾益脑片中梓醇的含量测定。
Objective: To establish a method for determination of catalpol in Bushenyiren tablets. Methods: Amethyst C_ (18) -H column (4.6 mm × 150 mm, 5 μm) was determined by high performance liquid chromatography. The column temperature was at room temperature. The mobile phase was acetonitrile-0.1% phosphoric acid solution 0.8 m L / min, detection wavelength is 205 nm. Results: Catalpol in Bushenyiren tablets had a good linear relationship with the peak area in the linear range of 2 ~ 100μg / m L with a correlation coefficient of 0.999 6 and an average recovery rate of 95.8% (n = 5) with RSD of 2.15%. Conclusion: The method is simple and rapid and can be used for the determination of catalpol in Bushen Yisue Tablets.