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目的 建立测定可溶性 CD137(s CD137)的酶联免疫吸附测定 (EL ISA)方法 ,研究 s CD137释放与 T细胞活化及CD137表达之间的相关性 ,探讨类风湿性关节炎 (RA)患者血清 s CD137测定的意义。 方法 建立生物素 -亲和素双抗体夹心 EL ISA(BA- EL ISA)方法检测血清 s CD137含量。用
Objective To establish a method for the determination of soluble CD137 (sCD137) by enzyme linked immunosorbent assay (ELISA) to study the correlation between s CD137 release and T cell activation and CD137 expression, and to explore the relationship between serum s Significance of CD137 assay. Methods The level of serum CD137 in serum was detected by the method of sandwich enzyme-linked immunosorbent assay (ELISA). use