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背景:流行病学研究显示,吸烟和帕金森病(Parkinson’sdisease,PD)存在负相关系,吸烟可能对PD具有保护作用。人们一直在探索尼古丁对PD的保护作用机制。目的:研究尼古丁对PD大鼠纹状体脑胶质源性神经营养因子(glialcelltinederivedneurotrophicfactor,GDNF)和多巴胺含量的影响。设计:随机对照研究。地点和对象:实验地点:华中科技大学同济医学院协和医院,80只大鼠被随机分为预防组50只和治疗组30只。干预:本文第一作者将6-羟多巴胺(6-hydroxydopamine,6-OHDA)立体定向注射到大鼠右侧中脑腹侧被盖部和黑质致密部,建立大鼠模型。采用生化,免疫组织化学方法观察不同剂量尼古丁对PD大鼠的作用。主要观察指标:检测纹状体GDNF表达及多巴胺含量的变化。结果:造模前及造模后皮下注射尼古丁的PD大鼠,纹状体GDNF表达及多巴胺含量较PD组有明显改善(P<0.05)。结论:尼古丁可减轻6-OHDA对黑质多巴胺能神经元的损伤,对PD大鼠具有保护作用。
BACKGROUND: Epidemiological studies have shown that there is a negative correlation between smoking and Parkinson’s disease (PD), and smoking may have a protective effect on PD. People have been exploring the protective effect of nicotine on PD mechanism. Objective: To study the effect of nicotine on glialcelltine-derived neurotrophic factor (GDNF) and dopamine content in striatum of PD rats. Design: Randomized controlled study. Location and Subjects: Experimental Location: Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 80 rats were randomly divided into prevention group 50 and treatment group 30. Intervention: The first author of this paper stereotactically injected 6-hydroxydopamine (6-OHDA) into the ventral tegmental area of the rat’s midbrain and substantia nigra to establish a rat model. The biochemical and immunohistochemical methods were used to observe the effects of different doses of nicotine on PD rats. MAIN OUTCOME MEASURES: Detection of GDNF expression in the striatum and changes in dopamine content. Results: GDNF expression and dopamine content of striatum were significantly improved in PD rats injected with nicotine subcutaneously before and after modeling (P <0.05). CONCLUSIONS: Nicotine can reduce the damage of substantia nigra dopaminergic neurons by 6-OHDA, and has a protective effect on PD rats.