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目的观察芹灵冲剂（ＱｉｎｌｉｎｇＣｈｏｎｇｊｉ，ＱＬ）对鸭体内鸭乙型肝炎病毒（ＤＨＢＶ）－ＤＮＡ的抑制效果方法待ＤＨＢＶ感染雏鸭７ｄ血清检测为阳性后，随机分组进行药物治疗试验，每组５只，ＱＬ分为高、中、低３个剂量组，分别为１０，５和２．５ｇ／ｋｇ组，ｉｇ，２次／ｄ，１０ｄ设病毒对照组（ＤＨ－ＢＶ），以生理盐水代替药物，ｉｇ，Ｂｉｄｘ１０．阳性药用无环鸟昔（ＡＣＶ）粉剂（２５ｍｇ／支），ｉｇ１００ｍｇ／ｋｇ，２次／ｄ，１０ｄ（Ｂｉｄｘ１０）在感染后７ｄ即用药前（Ｔ０），用药５ｄ（Ｔ５），用药１０ｄ（Ｔ１０）和停药后３ｄ（Ｐ３），自鸭腿经静脉取血，分离血清，－７０℃保存待检。结果ＱＬ（５，１０ｇ／ｋｇ）组，对雏鸭无毒性，给药５ｄ（Ｔ）和１０ｄ（Ｔ１０）能显著降低ＤＨＢＶ感染鸭血清中ＤＨＢＶ－ＤＮＡ的水平，其抑制率分别与病毒对照组比较，均有显著性差异（Ｐ＜０．０５和Ｐ＜０．０１）；低剂量组（２．５ｇ／ｋｇ）对鸭血清ＤＨＢＶ－ＤＮＡ也有一定的疗效３批实验结果一致，并有明显的量效关系结论ＱＬ体内有抗ＤＨＢＶ的作用，值得临床进一步研究．
Objective To observe the inhibitory effect of Qinling Chongji (QL) on Duck Hepatitis B virus (DHBV) -DNA in ducklings.Methods Seven days after DHBV infected ducklings were tested positive, the rats were randomly divided into five groups , QL was divided into high, medium and low doses of 3 groups, respectively 10,5 and 2.5g / kg group, ig, 2 times / d, 10d viral control group (DH-BV), with saline instead of drugs , Ig, Bidx10. The mice were sacrificed at 7 days after infection (T0), 5 days (T5) and 10 days (10 days) with positive drug ACV (25mg / kg), ig100mg / T10) and 3d after stopping (P3). Blood was collected from the calves through the vein of the duck leg, and the serum was separated and stored at -70 ℃ for examination. Results The QL (5 and 10g / kg) groups were non-toxic to ducklings. The levels of DHBV-DNA in DHBV-infected duck ducks at 5d (T) and 10d (T10) (P <0.05 and P <0.01). The low dose group (2.5g / kg) also had some effects on duck serum DHBV-DNA. The results of three batches of experiments were consistent and obvious Dose-effect relationship Conclusion QL in vivo anti-DHBV role, it is worth further clinical study.