论文部分内容阅读
以细胞培养、流式细胞仪ＤＮＡ含量分析及ＤＮＡ琼脂糖凝胶电泳方法研究了我国食管癌高发区磁县居民粮食中优势污染霉菌毒素脱氧雪腐镰刀菌烯醇（ＤＯＮ）和黄曲霉毒素Ｇ１（ＡＦＧ１）对人淋巴细胞凋亡的影响。流式细胞术（ＦＣＭ）检测结果显示，ＤＯＮ、ＡＦＧ１处理组淋巴细胞出现了典型的亚二倍体凋亡细胞峰，凋亡百分率与毒素作用时间（ＤＯＮ：２～７２小时；ＡＦＧ１：２～２４小时）及剂量（ＤＯＮ：５０～２０００μｇ／Ｌ；ＡＦＧ１：３．１２～２０００μｇ／Ｌ）呈正相关关系。ＤＮＡ琼脂糖凝胶电泳结果表明，ＤＯＮ（１０００μｇ／Ｌ）、ＡＦＧ１（１０００μｇ／Ｌ）处理２４小时淋巴细胞出现特征性的ＤＮＡ＂Ｌａｄｄｅｒ＂条带。因此表明，ＤＯＮ、ＡＦＧ１可诱导和促进体外培养的人外周血淋巴细胞发生凋亡。
Cell culture, flow cytometry DNA content analysis and DNA agarose gel electrophoresis were used to study the predominant contamination of mycotoxin deoxynivalenol (DON) and aflatoxin G1 in the prefecture of high incidence of esophageal cancer in China. (AFG1) Effects on apoptosis of human lymphocytes. Flow cytometry (FCM) results showed that the lymphocytes of DON and AFG1 treated groups showed typical subdiploid apoptotic cell peaks, and the percentage of apoptotic cells and toxin action time (DON: 2 to 72 hours; AFG1: 2 to 24 hours) and dose (DON: 50-2000 μg/L; AFG1: 3.12-2000 μg/L) were positively correlated. The result of DNA agarose gel electrophoresis showed that characteristic DNA “Ladder” bands were detected in lymphocytes treated with DON (1000 μg/L) and AFG1 (1000 μg/L) for 24 hours. Therefore, it was shown that DON and AFG1 can induce and promote the apoptosis of human peripheral blood lymphocytes cultured in vitro.