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目的研究普通小鼠和GCPⅡ基因剔除后小鼠脑外伤后脑水肿的变化,探讨并研究GCPⅡ基因剔除后对脑外伤后脑水肿的影响。方法雄性C57BL/6J小鼠随机20只,随机分为两组(C57BL/6J小鼠假手术组10只,C57BL/6J小鼠颅脑损伤组10只)。雄性GCPⅡ基因剔除小鼠随机20只,随机分为两组(GCPⅡ基因剔除小鼠假手术组10只,GCPⅡ基因剔除小鼠颅脑损伤组10只),应用PinPointTMPCI3000精细颅脑撞击仪,设定参数(打击深度为1.5mm;打击时间为80ms,打击的速度为1.5m/s),精确撞击小鼠脑皮质。伤后24h,行干湿重法测脑组织含水量,Western Blot检测AQP4蛋白的表达,MRI检测水肿程度,伊文思蓝检测血脑屏障破坏程度。结果GCPⅡ基因剔除小鼠较C57BL/6J小鼠外伤后神经功能明显改善(P<0.05);GCPⅡ基因剔除小鼠脑组织含水量、水肿体积以及外伤区域T2 WI信号强度较C57BL/6J小鼠降低(P<0.05)。结论小鼠的GCPⅡ基因剔除后脑外伤后脑水肿较C57BL/6J小鼠明显减轻,进一步证明小鼠GCPⅡ基因敲除后对脑外伤后脑组织的保护作用。
Objective To investigate the changes of brain edema after traumatic brain injury in mice and GCPⅡ knockout mice and to investigate the effect of GCPⅡ gene knockout on brain edema after traumatic brain injury. Methods Twenty male C57BL / 6J mice were randomly divided into two groups (10 in C57BL / 6J sham operation group and 10 in C57BL / 6J brain injury group). Twenty male GCPⅡ knockout mice were randomly divided into two groups (10 in GCPⅡ knockout mice sham operation group and 10 in GCPⅡ knockout mice brain injury group). Using PinPoint TMPCI 3000 precision cranial impact instrument, Parameters (combat depth of 1.5mm; strike time of 80ms, hit the speed of 1.5m / s), accurate impact on mouse cerebral cortex. 24h after injury, the water content of brain tissue was measured by dry-wet method, the expression of AQP4 protein was detected by Western Blot, the extent of edema was detected by MRI, and the damage of blood-brain barrier was detected by Evans blue. Results Compared with C57BL / 6J mice, the neurological function of GCPⅡ knockout mice was significantly improved after C57BL / 6J mice injury (P <0.05). Compared with C57BL / 6J mice, GCPⅡ knockout mice showed decreased water content, edema volume and T2 WI signal intensity (P <0.05). Conclusions The brain edema after GCPⅡ gene knockout in mice is significantly reduced compared with C57BL / 6J mice, which further proves the protective effect of GCP Ⅱ gene knockout on brain tissue after traumatic brain injury.