为选择一种快速、价廉,甚至可从单个胎儿细胞产前诊断13三体征的方法,作者采用了多态小串联重复(STRs)荧光聚合酶链反应(PCR)方法。 脱氧核糖核酸(DNA)准备:采集1 ml羊水细胞离心15 000 rpm/min 10 min,除去上清液,从Ready Amp Genomic纯化系统取100μl树脂加入细胞团。作为以后提取过程备用,每个PCR从这些DNA溶液中取3μl供使用。荧光PCR:PCR扩增是在两个分开 To select a rapid, inexpensive and even prenatal diagnosis of trisomy 13 from a single fetal cell line, the authors used a polymorphic, small tandem repeat (STRs) fluorescent polymerase chain reaction (PCR) method. DNA Preparation: Collect 1 ml of amniotic fluid and centrifuge at 15 000 rpm for 10 min. Remove the supernatant and add 100 μl of resin from the Ready Amp Genomic purification system to the cell pellet. As a spare for subsequent extraction, 3 μl of each of these DNA solutions were used for each PCR. Fluorescent PCR: PCR amplification is in two separate