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针对啤酒大麦花药培养中低温预处理方法、脱分化培养基及分化培养基中激素、添加物的种类、配比以及生根培养中提高糖浓度等进行大胆尝试，结果表明：１．直接接种花药于诱导培养基上进行低温预处理的方法可使花药反应频率及绿苗再生力得到改善。２．２，４Ｄ与６ＢＡ配合使用较２，４Ｄ与ＺＴ的配合脱分化作用强烈。３．提高２，４Ｄ和６ＢＡ的浓度有助于提高花药反应频率，改善愈伤质量，并对绿苗分化率无不良影响。４．５０μｍｏｌ·Ｌ－１的５氮胞苷能促进花药脱分化产生大量愈伤组织。５．在诱导和分化培养中补加酵母浸提物可使花药启动反应加强，增加绿色再生植株数量。６．生根壮苗培养中采用高蔗糖浓度能够明显改善绿苗生长状态，使其分蘖增加，生长旺盛，移栽易成活。
Aiming at the low temperature pretreatment method of beer barley anther culture, the hormone, the kinds and proportion of hormone in the dedifferentiation medium and differentiation medium, and the increase of sugar concentration in rooting culture, the experiment was made boldly. The results showed as follows: 1. Direct inoculation of anther on induction medium for low temperature pretreatment method can make the anther reaction frequency and green shoot regeneration improved. 2.2,4 D and 6 BA with the use of 2,4 D compared with ZT dedifferentiation strongly. 3. Increasing the concentrations of 2,4D and 6BA can improve the frequency of anther reaction, improve the callus quality and have no adverse effect on the rate of green plantlet differentiation. 4. 5μmol·L-1 5-azacytidine can promote dedifferentiation of anther to produce a large number of callus. 5. Supplementation of yeast extract in induction and differentiation culture can enhance anther priming reaction and increase the number of green regenerated plants. 6. Strong rooting seedling culture using high sucrose concentration can significantly improve the green seedling growth status, increase its tillering, strong growth, transplanting easy to survive.