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为揭示小麦-柔软滨麦草易位系M853-4的抗条锈性遗传机制,以易位系M853-4和感病品种铭贤169为亲本制备F2、F3代种子,采用人工接种的方法于温室中接种小麦条锈菌生理小种Su-11,用于测定M853-4及其杂交后代的苗期抗条锈性。结果表明,M853-4对Su-11的抗病性由1对显性和1对隐性基因控制。筛选由1对显性基因控制的F3代分离家系作为SSR标记群体,从320对引物中共找到了4个位于4A染色体上的与该显性基因(暂命名为YrLm2)紧密连锁的微卫星标记Xgwm44、Xwmc650、Barc170和Xwmc718,标记到YrLm2的遗传距离分别为15.0、5.0、3.9和3.1cM,并将YrLm2定位于4A染色体的长臂上,标记结果可用于小麦分子辅助育种。
In order to reveal the genetic mechanism of resistance to stripe rust in wheat-soft hamamatism translocation line M853-4, F2 and F3 generations of seeds were prepared using the translocation line M853-4 and the susceptible variety Mingxian 169 as seeds, and artificial inoculation Inoculation of wheat stripe rust physiological races Su-11 in the greenhouse was used to determine the stripe rust resistance of M853-4 and its hybrid progenies at seedling stage. The results showed that the resistance of M853-4 to Su-11 was controlled by 1 dominant and 1 recessive gene. Screening F3 families separated from one dominant gene as SSR markers, we found 4 microsatellite markers Xgwm44 (located temporarily on chromosome 4A) closely linked to this dominant gene (tentatively named YrLm2) from 320 pairs of primers , Xwmc650, Barc170 and Xwmc718. The genetic distance to YrLm2 was 15.0, 5.0, 3.9 and 3.1 cM, respectively, and YrLm2 was located on the long arm of chromosome 4A. The marker results could be used for molecular breeding assisted by wheat.