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目的探讨长期摄入高脂不同n-3/n-6多不饱和脂肪酸(PUFAs)构成比的饮食后,大鼠胰岛素敏感性及血清炎症因子表达水平的变化。方法 40只刚断乳雄性SD大鼠适应性喂养7天后,根据体重随机分为4组:空白对照组(基础饲料)、高脂组(猪油)、高脂1∶1组(n-3/n-6为1∶1)和高脂1∶4组(n-3/n-6为1∶4),每组10只。每周记录一次大鼠体重,喂养16周处死动物,检测大鼠血脂、血清胰岛素敏感性和血清炎症因子(IL-6、TNF-α和hs-CRP)表达水平。结果与空白对照组相比,其他3个组体重显著上升(P<0.05);高脂1∶1组胰岛素敏感性与空白对照组比较,差异无显著性,且显著高于高脂组和高脂1∶4组(P<0.05);与高脂组相比,高脂1∶1组血清TNF-α和hs-CRP表达水平显著下降(P<0.05)。结论长期摄取高多不饱和脂肪酸同样具有肥胖风险,提高n-3PUFAs在膳食构成中的比重可以有效抑制炎症因子表达,改善胰岛素敏感性,预防胰岛素抵抗的发生。
Objective To investigate the changes of insulin sensitivity and serum inflammatory cytokines in rats fed with high fat diet containing different proportions of n-3 / n-6 polyunsaturated fatty acids (PUFAs). Methods Forty freshly weaned male Sprague-Dawley male rats were fed for 7 days. The rats were randomly divided into 4 groups according to body weight: control group (basal diet), high fat group (lard), high fat group 1: 1 / n-6 is 1: 1) and high-fat 1: 4 group (n-3 / n-6 is 1: 4) The body weight of rats was recorded once a week. Animals were sacrificed 16 weeks after feeding. The levels of serum lipids, serum insulin and serum inflammatory cytokines (IL-6, TNF-α and hs-CRP) were measured. Results Compared with the blank control group, the body weight of the other three groups increased significantly (P <0.05). There was no significant difference in the insulin sensitivity between the high-fat 1 and the 1 group and the blank control group (P <0.05). Compared with hyperlipidemia group, the levels of serum TNF-αand hs-CRP in HF group were significantly decreased (P <0.05). Conclusion Long-term intake of polyunsaturated fatty acids is also at risk of obesity. Increasing the proportion of n-3 PUFAs in dietary composition can effectively inhibit the expression of inflammatory factors, improve insulin sensitivity and prevent the occurrence of insulin resistance.