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目的采用超快速液相色谱(UFLC)法同时测定龙胆中龙胆苦苷、獐芽菜苦苷和獐芽菜苷的含量。方法采用Shim-Pack XR-ODS柱(75 mm×3.0 mm,2.2μm),流动相为甲醇-0.1%磷酸水溶液(梯度洗脱),检测波长为240 nm,流速为0.5 m L·min~(-1),柱温为40℃。结果龙胆苦苷、獐芽菜苦苷和獐芽菜苷进样量分别在1.0~20μg(r=0.9998)、1.0~20μg(r=0.9995)、0.12~2.4μg(r=0.9996)范围内与峰面积呈良好的线性关系,平均加样回收率分别为97.3%、99.3%和98.3%(n=3),RSD均小于1.0%。结论 UFLC法简便、快速、准确、重现性好,可用于龙胆药材中环烯醚萜苷类成分的质量控制研究。
OBJECTIVE To determine the contents of gentiopicroside, swertia glycosides and mesogenin in gentian by ultra-fast liquid chromatography (UFLC). Methods A Shim-Pack XR-ODS column (75 mm × 3.0 mm, 2.2 μm) was used with a mobile phase of methanol-0.1% phosphoric acid (gradient elution). The detection wavelength was 240 nm and the flow rate was 0.5 m L · min ~ -1) and the column temperature was 40 ° C. Results The injection rates of gentiopicroside, swertia glycoside and romaine were between 1.0 ~ 20μg (r = 0.9998), 1.0 ~ 20μg (r = 0.9995), 0.12 ~ 2.4μg The average recovery was 97.3%, 99.3% and 98.3% (n = 3), respectively. The RSDs were all less than 1.0%. Conclusion The UFLC method is simple, rapid, accurate and reproducible. It can be used for quality control of iridoid glycosides in gentian.