【摘 要】
：
Dendrobium officinale is not only an ornamental plant,but also a valuable medicinal herb that is widely used in traditional Chinese medicine.However,distinguishing D.officinale from other Dendrobium species is usually a difficult task.In this study,we dev
【机 构】
：
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
论文部分内容阅读
Dendrobium officinale is not only an ornamental plant,but also a valuable medicinal herb that is widely used in traditional Chinese medicine.However,distinguishing D.officinale from other Dendrobium species is usually a difficult task.In this study,we developed a rapid identification protocol for D.officinale using the loop-mediated isothermal amplification (LAMP) method.A set of primers were specifically designed to detect a modified internal transcribed spacer region of D.officinale at 65 ℃ within 40 min after adding SYBR(R) Green I,which was used for the detection of D.officinale.Unlike commonly used adulterants,reaction mixtures containing D.officinale DNA changed from orange to green,and this color change was easily observed with the naked eye.Thus,this methodology can be used to accurately differentiate D.officinale from other Dendrobium species,is quick as all D.officinale samples were amplified within 40 min,and specific as samples of the adulterants were not amplified.The specificity of this LAMP-based method was confirmed by testing 17 samples of D.officinale and 32 adulterant samples from other Dendrobium species.This LAMP-based rapid identification method does not require expensive equipment or specialized techniques and can be used in field surveys for accurate and fast on-site identification.
其他文献
Four new corynanthe-type alkaloids,meloslines C F (1-4),together with four known ones (5-8) were isolated from the roots of Alstonia scholaris.Their structures including absolute configurations were elucidated by extensive spectroscopic analysis and elect
Anemoneflaccida Ft.Schmidt is a perennial medicinal herb that contains pentacyclic triterpenoid saponins as the major bioactive constituents.In China,the rhizomes are used as treatments for a variety of ailments including arthritis.However,yields of the s
Bipolarins A-H (1-8),eight new tetracyclic ophiobolin-type sesterterpenes featuring a rare oxaspiro[4.4]nonane moiety,were isolated from cultures of fungus Bipolaris sp.TJ403-B1.Their structures and absolute configurations were elucidated by comprehensive
Multidrug resistance (MDR) is one of the major obstacles in cancer chemotherapy.Our previous study has shown that icariin could reverse MDR in MG-63 doxorubicin-resistant (MG-63/DOX) cells.It is reported that icariin is usually metabolized to icariside Ⅱ
Metastasis is responsible for the majority of cancer-related deaths and prevention of metastasis remains a big challenge for cancer therapy.Cucurbitacin B (Cuc B) is a natural triterpenoid with potent anticancer activities while its effect on metastasis r
Two new folate-derived analogues,named uncarophyllofolic acids A (1) and B (2),respectively,were isolated from the Uncaria rhynchophylla hook bearing stem (Gouteng in Chinese).The distinct stereochemical structures of 1 and 2 were determined by spectrosco
The cornerstone of antimalarial treatment,artemisinin,has reduced malaria associated morbidity and mortality worldwide.However,Plasmodium falciparum parasites with reduced sensitivity to artemisinin have emerged,and this threatens malaria control and elim
A new sesquiterpenoid and two pregnane steroids,named vemobockolide C (1) and vernobockones A and B (2 and 3),respevtively,along with a known sesquiterpenoid,7,10-epoxy-ll-hydroxy-bisabol-2-en-15-al,were isolated from the aerial part of Vernonia bockiana.
The underground cane of Schizocapsa plantaginea (Hance) has long been used by Chinese ethnic minority as a constituent of anti-cancer formulae.Saponins are abundant secondary metabolic products located in the underground cane of this plant.The potential t
Stellera chamaejasme L.is a traditional Chinese medicine with a long history to treat stubborn skin ulcer,and it also has antiviral and antitumor effects.Neochamaejasmine B (NCB),Neochamaejasmine A (NCA) and Chamaechromone (CMC) are the major components i