论文部分内容阅读
Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from three weeks after pollination. (2) The germinationand growth of embryos were different at different growth stages, which could germinateand grow with PF value>0.5, but failed with PF value<0.5. In embryo rescue system ofhybrids, the best germination and differentiation medium was MS+6-BA 2mgL-1+IAA 0.3mgL-1,the rate of germination and differentiation reached up to 80%, bud induction andmultiplication medium was MS+6-BA 1.5mgL-1+IAA 0.3mgL-1, rooting medium was 1/2 MS+IAA0.8mgL-1. Some hybrids were transplanted into the field successfully. (3) Leaf shapeinvestigation and identification by S allele-specific PCR and RAPDs showed that thehybrids were true ones.
Embryo abortion stage and rescue system of hybrids were studied in the distant hybridization between plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from three weeks after pollination. (2) The germination and growth of embryos were different in different growth stages, which could germinate and grow with PF value> 0.5, but failed with PF value <0.5. In embryo rescue system of hybrids, the best germination and differentiation medium was MS + 6-BA 2 mgL- 1 + IAA 0.3 mg L- 1, the rate of germination and differentiation reached up to 80%, bud induction and multiplication medium was MS + 6-BA 1.5 mgL -1 + IAA 0.3 mg L -1, rooting medium was 1/2 MS + IAA 0.8 mg L -1. Some hybrids were transplanted into the field successfully. (3) Leaf shape investigation and identification by S allele-specific PCR and RAPDs showed that the hybrids were true ones.