site-directed相关论文
Uncarialins J-M from Uncaria rhynchophylla and Their Anti-depression Mechanism in Unpredictable Chro
Main observation and conclusionrnUncaria rhynchophylla has been widely used to treat central nervous system diseases for......
Important amino acid in the hemagglutinin glycoprotein of measles virus (MV) that governs hemadsorpt
Two strains of measles virus IMA and SMD that were isolated using B95a cell line show hemadsorption negative. After adap......
Construction and screening of a multi-point site- specific mutant library of subtilisin E with a set
A mutant library of subtilisin E containing random combinations of various mutagenized sites wasconstructed by one-round......
目的 观察5非编码区(5UTR)嘧啶富集区变异对B组柯萨奇病毒1型(CVB1)毒力和致病性的影响.方法 定点诱变将CVB1基因组5UTR第563～573碱......
本研究采用点突变PCR方法成功克隆并表达了绵羊肺炎支原体(Mycoplasma ovipneumoiae,M.O)内蒙古分离株(SZWQ株)的黏附因子基因(SZW......
以大肠杆菌Ｋ１２菌标总ＤＮＡ为模声，利用技术扩增了编码天冬氨酸激酶的ｌｙｓＣ基因，序列分析结果表明，目的含有１３５０个核苷酸（包括起始密码和终止密码），与文献报......
细胞色素P450酶（P450）的分子改造及其催化的生物转化是当前的两个研究热点．本研究以扩展P4502A6的底物适应性为目标，通过尝试gCP4502A6......
背景：NOD2/CARD15基因序列单核苷酸多态性（SNP）与欧美人群的克罗恩病（CD）明显相关．其中R702W、G908R和3020insC3个SNP位点与CD的相关性尤......
利用重叠延伸PCR法对扩展青霉碱性脂肪酶(PEL)基因进行体外定点突变,并构建了含突变基因的重组质粒pPIC3.5K-lip-D92P.将该质粒在......
目的：研究B结构域糖基化位点对凝血因子Ⅷ分泌和活性的影响。方法：通过反向PCR技术在凝血因子ⅧA2和A3结构域之间插入B结构域的糖基......
采用定点突变技术,将人胰岛素原基因编码框中A11位氨基酸残基与A12位氨基酸残基进行了互换,构建了[CysA11Ser,SerA12Cys]人胰岛素......
目的：利用长距离PCR定点突变技术对GEFS^＋致病相关基因SCN1A进行定点诱变。方法：在突变位点处设计一对延伸方向相反的引物,通过长距离......
为使鸡IL-18成熟蛋白基因在真核系统中高效表达，对其进行定点突变，将酵母低频密码子突变为偏嗜性密码子，构建了鸡IL-18成熟蛋白变构基......
The lincosamide class of antibacterials is widely used for the treatment of a broad spectrum of infections, and one prev......
In this study, the optimum temperature was lowered while the residual phytase activity after heating to 70℃ was raised ......
背景与目的已有的研究证明nm23-H1基因是一个重要的肿瘤转移抑制基因，但其抑制肿瘤转移的生化机理尚不完全清楚。Nm23-H1基因结构和......
Monitoring the autoproteolysis of hiv-1 protease by site-directed spin-labeling and electron paramag
Site-directed spin-labeling with continuous wave electron paramagnetic resonance spectroscopy was used to monitor autopr......
Role of amino acid residues involved in the active cavity of proline iminopeptidase in catalytic act
The proline iminopeptidase (PchPiPA) of the white-rot fungi Phanerochaete chrysosporium is an exopeptidase specific to c......
The active site motif of proteins belonging to "Histidine Acid Phosphatase" (HAP) contains a hepta-peptide region, RHGXR......
目的构建R617C、P508L和G512S突变型表达载体并对其进行鉴定。方法以野生型pGEX-4T-2-NBD原核表达载体为模板，通过体外诱导突变构建......
Structural analysis of Candida antarctica lipase B (CALB) indicates that side chain of leucine at 278 site lies above th......
