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猪瘟病毒能导致猪的高度接触性传染病,对养猪业危害极大。由于传统猪瘟疫苗在使用及生产中存在诸多不足,促使科研工作者研制开发新型猪瘟疫苗。猪瘟病毒主要保护性抗原是E2蛋白和Erns蛋白,能够引起机体的免疫反应。植物表达体系具有表达效果好和生产成本低等优点,随着近些年开始出现利用转基因植物生产蛋白产品的技术,植物生物反应器越来越引起广泛关注。植物生物反应器具有完整的真核细胞表达系统,能够进行准确的蛋白翻译后加工,能较好的保留蛋白生物活性。为利用转基因植物生物反应器制备猪瘟的亚单位可饲疫苗及其商品化提供实验依据,本研究拟构建种子特异性启动子驱动的目的基因的表达载体,并转导至亚麻芥生物反应器中,表达猪瘟病毒E2-Erns融合蛋白。利用表达的猪瘟病毒E2-Erns蛋白以口服接种的方式进行小鼠模型的免疫实验,验证目标蛋白的抗原性。
Classical swine fever virus can cause highly contagious diseases of swine and is very harmful to pig industry. As the traditional swine fever vaccine in the use and production there are many deficiencies, prompting research workers to develop new strains of classical swine fever vaccine. The main protective antigen of classical swine fever virus is E2 protein and Erns protein, which can cause the body’s immune response. The plant expression system has the advantages of good expression effect and low production cost, and plant bioreactor attracts more and more attention with the technology of producing protein products using transgenic plants in recent years. Plant bioreactor with a complete eukaryotic expression system, capable of accurate protein post-translational processing, can better retain the biological activity of the protein. In order to provide experiment evidences for the production of swine fever subunit vaccine and its commercialization by using transgenic plant bioreactor, we constructed the expression vector of seed-specific promoter-driven gene and transferred it to Flax mustard bioreactor , The classical swine fever virus E2-Erns fusion protein was expressed. The expression of the swine fever virus E2-Erns protein oral inoculation of the mouse model of immune experiments to verify the antigenicity of the target protein.