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目的:研究含蛋白的不对称膜高分子囊泡包封进PLGA微球后对其体外释放动力学的改善作用。方法:将包封有BSA蛋白的不对称膜高分子囊泡采用S/O/W法包裹进PLGA微球中,制备复合微球,对微球表征后,以包封葡聚糖颗粒的微球做对照品,于37℃测定微球的体外释放,比较两者的释放曲线,考察不对称膜高分子囊泡对微球中蛋白释放的改善作用。结果:①经扫描电镜(SEM)观察,包裹高分子囊泡的复合微球形态圆整,表面光滑,平均粒径为75.20μm,粒径较为均匀,复合微球制备成功。②比较复合微球和对照微球的释放曲线,发现对照微球有较小的突释,而复合微球的几乎没有突释效应。结论:不对称膜高分子囊泡包封进PLGA微球后可以很好的改善蛋白的释放行为,获得更为理想的释放曲线。
OBJECTIVE: To study the effect of improving the release kinetics of PLGA microspheres containing protein asymmetric membrane vesicles. Methods: Asymmetric membrane vesicles coated with BSA protein were encapsulated in PLGA microspheres by S / O / W method to prepare composite microspheres. After characterizing the microspheres, Ball as a reference substance, the release of microspheres in vitro was measured at 37 ℃. The release curves of the two microspheres were compared to investigate the effect of asymmetric membrane vesicles on the protein release in the microspheres. Results: ①The morphology of the composite microspheres encapsulating the macromolecular vesicles was observed by scanning electron microscopy (SEM). The morphology of the composite microspheres was smooth and the surface was smooth. The average diameter was 75.20μm. The size of the composite microspheres was uniform. The composite microspheres were successfully prepared. ② Compared with the release curve of the composite microspheres and the control microspheres, it was found that the control microspheres have a smaller burst release, while the composite microspheres almost no burst effect. CONCLUSION: Asymmetric membrane vesicles encapsulated in PLGA microspheres can improve the release behavior of protein and obtain a more ideal release curve.