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目的观察曲古抑菌素A(Trichostatin A,TSA)对甲状腺鳞癌SW579细胞株Akt、mTOR和p21基因表达的影响,探讨TSA抗甲状腺癌的作用机制。方法体外培养SW579细胞,用CCK-8法检测TSA对SW579的生长抑制作用,计算半数抑制浓度。实验分为对照组、TSA组、Wortmannin组、Wortmannin+TSA组、Rapamycin组,用RT-PCR方法检测SW579细胞Akt、p21及mTOR的mRNA表达,分析各处理组Akt、p21及mTOR基因表达变化。结果 CCK-8结果显示,TSA可明显抑制SW579的增殖,并呈剂量依赖性,半数抑制浓度约为147nmol/L。RT-PCR检测结果表明,与对照组相比,TSA组、Wortmannin组和Wortmannin+TSA组Akt、mTOR mRNA表达水平明显降低,TSA组p21表达显著上调,Wortmannin组p21表达明显降低,各组mTOR表达均明显降低。结论 TSA在一定浓度范围内对甲状腺鳞癌细胞株SW579有剂量依赖性的增殖抑制作用,其机制可能与TSA降低SW579细胞Akt、mTOR基因的表达,进而再上调p21的表达有关。
Objective To investigate the effect of trichostatin A (TSA) on the expression of Akt, mTOR and p21 in thyroid squamous cell carcinoma SW579 cell line and to explore the mechanism of TSA anti-thyroid cancer. Methods SW579 cells were cultured in vitro. The growth inhibitory effect of TSA on SW579 cells was detected by CCK-8 assay and the half inhibitory concentration was calculated. The experimental groups were divided into control group, TSA group, Wortmannin group, Wortmannin + TSA group and Rapamycin group. The mRNA expressions of Akt, p21 and mTOR in SW579 cells were detected by RT-PCR and the expressions of Akt, p21 and mTOR were analyzed. Results The results of CCK-8 showed that TSA significantly inhibited the proliferation of SW579 in a dose-dependent manner with a median inhibitory concentration of 147 nmol / L. The results of RT-PCR showed that the expression of Akt and mTOR mRNA in TSA group, Wortmannin group and Wortmannin + TSA group were significantly lower than those in control group, the expression of p21 in TSA group was significantly increased, and the expression of p21 in Wortmannin group was significantly decreased Significantly lower. Conclusions TSA can inhibit the proliferation of SW579 cells in a dose-dependent manner. TSA can reduce the expression of Akt and mTOR in SW579 cells, and then up-regulate the expression of p21.