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目的：利用气相色谱分析法对根面龋牙本质标本中的有机酸作定性定量测定。方法：３０ 名患者（ 年龄４５ ～７８ 岁）的４４ 个牙齿的根面龋牙本质标本，以２５ｇ／Ｌ 偏磷酸溶解，低温离心（１０ ０００ｒ／ｍｉｎ，４ ℃，１０ｍｉｎ），上清液作气相色谱分析。结果：大多数根面龋标本中均检测到乳酸、甲酸、乙酸、丙酸和丁酸，少数标本中还检测到戊酸。各有机酸含量由高到低依次为甲酸（１９ ．１１±４ ．７１）ｇ／Ｌ；乙酸（２ ．７６ ±１ ．３８）ｇ／Ｌ；丙酸（１．１８ ±０ ．８４）ｇ／Ｌ；乳酸（１ ．０５ ±１．２）ｇ／Ｌ；丁酸（０ ．８１ ±０ ．４２）ｇ／Ｌ；戊酸（０．０４ ±０．１５）ｇ／Ｌ。结论：根面龋中的有机酸以甲酸、乙酸和丙酸为主，占各有机酸含量的９２ ．４ ％ ，主要是细菌利用龋损中胶原等内源性物质而产生的。
Objective: To qualitatively and quantitatively determine organic acids in root carious dentine by gas chromatography. METHODS: Root caries of 44 patients (aged 45-78 years) with 30 teeth were dissolved in 25 g / L metaphosphoric acid and centrifuged at low temperature (10 000 r / min, 4 ° C, 10 min). The supernatant Gas chromatographic analysis. RESULTS: Lactic acid, formic acid, acetic acid, propionic acid, and butyric acid were detected in most root caries samples, and valeric acid was also detected in a few samples. The contents of organic acids from high to low were as follows: formic acid (19.11 ± 4.71) g / L; acetic acid (2.76 ± 1.38) g / L; propionic acid (1.18 ± 0.84) g / L; lactic acid (1.05 ± 1.2) g / L; butyric acid (0.81 ± 0.42) g / L; pentanoic acid (0.04 ± 0.15) g / L. Conclusion: The organic acids in root caries are dominated by formic acid, acetic acid and propionic acid, accounting for 92% of the total organic acids. 4%, mainly bacterial use of collagen and other endogenous substances in carious lesions generated.