大豆皂甙是从大豆中提取的糖苷,在未加工的大豆中含量为5.6％。目前,国内外已开始重视对大豆皂甙的研究,但有关大豆皂甙的致突变研究报道较少。本文利用常规突变试验方法——鼠伤寒沙门氏菌/微粒体试验(Ames试验),探讨大豆皂甙的致突变性。 本实验所用方法为平板掺入法,预先对四种菌株TA_(97)、TA_(98)、TA_(100)、TA_(102)做6项鉴定,即组氨酸营养缺陷鉴定、深粗糙突变鉴定、ΔuVrB鉴定、R因子鉴定、PAQI质粒鉴定、自发回变鉴定,均符合要求。大豆皂甙由本校基础医学部提供。阳性对照物为敌克松(50μg/皿)、叠氮钠(1.5μ/皿)、丝裂霉素(0.5μg/皿)、2-乙酰氨基芴(10μg/皿)。实验用大鼠肝微粒体酶(S_9)用多氯联苯诱导。大豆皂甙每个浓度均做了3个平行皿,取均值。实验 Soybean saponin is a glycoside extracted from soybeans and contained 5.6% in unprocessed soybeans. At present, domestic and foreign researchers have begun to pay attention to the research of soybean saponin, but there are less reports on the mutation of soybean saponin. In this paper, the mutagenicity of soybean saponin was investigated using the conventional mutation test method, Salmonella typhimurium/microsome test (Ames test). The method used in this experiment was a plate incorporation method. Six types of TA_(97), TA_(98), TA_(100), and TA_(102) strains were identified in advance, namely histidine auxotrophy identification, and deep rough mutations. Identification, ΔuVrB identification, R factor identification, PAQI plasmid identification, and spontaneous reversion identification all met the requirements. Soya saponin is provided by the School of Basic Medicine. The positive controls were Dixon (50 μg/dish), Sodium Azide (1.5 μg/dish), Mitomycin (0.5 μg/dish), 2-Acetylaminoanthraquinone (10 μg/dish). Experimental rat liver microsomal enzyme (S_9) was induced with PCB. Soybean saponins were made into 3 parallel dishes at each concentration, taking the mean value. experiment