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据报道神经-内分泌-免疫系统之间有着密切的联系,神经系统在调节机体免疫功能方面起着极其重要的作用,定位刺激或损毁中枢神经系统的某些部位会对免疫功能产生不同的影响。为验证上述结论,我们通过外科手述选择性破坏大鼠左、右侧额叶,观察其对免疫功能的远期影响。1 实验方法1.1 动物分组 健康雄性Wistar大鼠61只,体重190～230g,随机分为4组:左侧额叶破坏组(A组)15只;右侧额叶破坏组(B组)18只;假手术组(C组)14只;无创伤对照组(D组)14只。1.2 动物处理 根据大鼠脑立体定向图谱,用微型电钻于前囱前方0～4mm、正中线左侧旁开2～5mm处钻孔,用双极电凝毁损3mm×2mm范围的脑组织,深度1.5mm,此为左额叶毁损组;右额叶毁损组动物在右侧相应部位操作。假手术组动物仅切开硬脑膜;无创伤对照组动物不作任何处理。术后第19天动物麻醉后心脏穿刺采血,断颅取脑。
It is reported that there is a close relationship between the neuroendocrine-immune system. The nervous system plays an extremely important role in regulating the immune function of the body. Locating or stimulating certain parts of the central nervous system may have different effects on immune function. To verify the above conclusion, we selectively destroy the left and right frontal lobes of rats by surgical procedures to observe their long-term effects on immune function. EXPERIMENTAL METHODS A. Animal groups Sixty-one male Wistar rats weighing 190-230 g were randomly divided into 4 groups: 15 in the left frontal lobe destruction group (group A), 18 in the right frontal lobe destruction group (group B) ; Sham operation group (group C) 14; non-invasive control group (group D) 14. 1.2 Animal Treatment According to rat brain stereotactic map with a mini drill in the front of the chimney in front of 0 ~ 4mm, left side of the median line next to open 2 ~ 5mm drilling, with bipolar coagulation damage 3mm × 2mm range of brain tissue, depth 1.5mm, this is the left frontal lobe damage group; the right frontal lobe damage the animals in the right part of the corresponding operation. Animals in sham operation group were only incised dura; animals in non-invasive control group were treated without any treatment. After the first 19 days of animal anesthesia cardiac puncture blood, broken cranial brain.