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目的建立新的转移性人肝细胞癌细胞系并研究其生物学特性。方法从人肝细胞癌的腹腔转移灶取材,将标本分离成单细胞悬液,使用培养液进行原代和传代培养,取名为HN-HC1细胞,观察细胞的形态,绘制细胞生长曲线。于裸小鼠腹腔接种第18代HN-HC1细胞2×106个,观察癌细胞的生物学特性,检测AFP的表达。结果 HN-HC1细胞体外连续传代至第18代,形态上具有典型的恶性上皮细胞的特征,裸小鼠腹腔HN-HC1细胞成瘤率100%,移植瘤细胞中AFP呈强阳性表达。结论 HN-HC1细胞可能成为较稳定的来源于转移灶的人肝细胞癌细胞系,HN-HC1裸小鼠移植瘤是一种较理想的肝细胞癌动物模型,为肝细胞癌的研究提供了良好的动物实验平台。
Objective To establish a new metastatic human hepatocellular carcinoma cell line and study its biological characteristics. Methods The peritoneal metastases from human hepatocellular carcinoma were harvested and the specimens were separated into single cell suspensions. Primary and subcultured cultures were used for culture. The cells were named as HN-HC1 cells. The morphology of the cells was observed and the cell growth curve was drawn. The nude mice were inoculated intraperitoneally with 2 × 106 HN-HC1 cells for 18 generations to observe the biological characteristics of the cancer cells and detect the expression of AFP. Results HN-HC1 cells were passaged continuously to the 18th passage in vitro. The morphologically typical HN-HC1 cells were characterized by malignant epithelial cells. The HN-HC1 cells in the nude mice had a tumorigenicity rate of 100%. AFP was strongly expressed in the transplanted tumor cells. Conclusion HN-HC1 cells may be a more stable human hepatocellular carcinoma cell line derived from metastasis. HN-HC1 nude mice xenografts are an ideal animal model of hepatocellular carcinoma and provide the basis for the study of hepatocellular carcinoma Good animal experiment platform.