对于高效液相色谱来说,为了提高分离度而不断加长色谱柱的办法是不可取的。这不仅是因为液相色谱柱的价格昂贵,而且是在色谱柱加长后,柱压升高.要求仪器的耐压程度更高.给仪器制作带来更多的困难。再循环色谱法可以在不增加色谱柱长的情况下提高分离度。它最初曾用于气相色谱,终因技术问题未能付诸实践.后来,由Porath For high-performance liquid chromatography, in order to improve the resolution and the continuous extension of the column approach is not desirable. This is not only because HPLC columns are expensive, but also because of the increased column pressure after the column is lengthened, requiring a higher degree of pressure resistance and more difficulties with instrument manufacture. Recirculation chromatography increases resolution without increasing the column length. It was originally used in gas chromatography and was eventually inoperable due to technical problems later developed by Porath