从养殖场排污口土壤样品中筛选得到7株具有四环素降解能力的菌株.其中菌Z-8具有较高的四环素降解活力,MIC实验显示,该菌株同时具有较高的耐四环素能力.通过形态学、分子生物学鉴定,菌Z-8为Sphingobacteriumsp.(鞘氨醇杆菌属).以葡萄糖为碳源、蛋白胨为氮源,30℃,初始pH 7.5,装液量为60mL/250mL,培养菌株四环素的降解率最高.降解条件研究显示:pH对四环素的降解率影响不大,最适降解温度为35℃. Seven strains of tetracycline-degrading bacteria were screened from soil samples collected from the sewage outfall of the farm, of which, strain Z-8 had higher tetracycline-degrading activity and MIC test showed that the strain had higher tetracycline- , Identified by molecular biology, and strain Z-8 was Sphingobacterium sp. Using glucose as carbon source and peptone as nitrogen source, initial strain of pH 7.5 and liquid volume of 60 mL / 250 mL were added at 30 ℃, The degradation rate was the highest.The study of degradation conditions showed that pH had little effect on the degradation rate of tetracycline and the optimum degradation temperature was 35 ℃.