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河蟹在蜕壳前,血淋巴中 20-羟基蜕皮酮(20-HE)含量达到最高峰——116.16±32.96ng/ml;蜕壳时降入低谷——7.09±1.39ng/ml;蜕壳后仍然很低。血淋巴中20-HE 调控着河蟹的蜕壳周期。雌性河蟹卵母细胞进入大生长期,9月份血淋巴中 17β-雌二醇(17β-E_2)出现峰值——3.62±1.11ng/ml,促进了卵母细胞的卵黄积累。雄性河蟹10月份血淋巴中睾酮(T)含量开始上升,至12月份达到 2.15±0.28ng/ml,诱导精巢发育成熟。雌性河蟹血淋巴中 20-HE 含量在5月和8月各出现一个峰值,分别为 13.15±6.99和 15.80±7.40ng/ml;雄性河蟹则在6月和9月各出现一个峰值,分别为 20.25±13.58和 14.00±9.48ng/ml。雌、雄河蟹同在2月份为全年最低值,分别为3.75±3.38 和3.68±1.78ng/ml。实验结果阐明了雌、雄河蟹 5、6月份血淋巴中 20-HE 出现的峰值与生长有关;8、9 月份出现的峰值与青春蜕壳、早期性腺发育相联系。
In the crabs, the 20-hydroxyecdysone (20-HE) reached the highest peak in the hemolymph before decidua - 116.16 ± 32.96ng / ml; it dropped to the lowest at molt - 7.09 ± 1.39ng / ml; Still low. 20-HE in the haemolymph regulates the molting cycle of crabs. Female crab oocytes entered the large growth phase, and the peak value of 17β-E2 appeared in the hemolymph in September (3.62 ± 1.11 ng / ml), which promoted the egg yolk accumulation in oocytes. Testosterone (T) levels in the hemolymph of male crabs began to rise in October, reaching 2.15 ± 0.28 ng / ml in December, which induced the development of mature testes. The content of 20-HE in haemolymph of female crab showed a peak at 13.15 ± 6.99 and 15.80 ± 7.40ng / ml respectively in May and August, while the male crab showed a peak at June and September respectively, which was 20.25 ± 13.58 and 14.00 ± 9.48 ng / ml. Female and male crabs in February the lowest for the year, were 3.75 ± 3.38 and 3.68 ± 1.78ng / ml. The results of the experiment show that the peak value of 20-HE appeared in the hemolymph of female and male crabs in May and June was related to the growth; the peak appeared in August and September was related to the development of young molt and early gonadal development.