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目的 :为克痨星胶囊中黄芪的含量测定建立质控标准。方法 :将黄芪中的环黄芪醇类皂甙用 1%的醇制氢氧化钾皂化 ,使糖部分的乙酰基均转变为羟基 ,使环黄芪醇类皂甙均转变为黄芪甲甙 ,以HPLC方法进行检测。HPLC条件 :Waters公司NovapakC18( 3.9mm× 15 0mm)色谱柱 ,40 %甲醇为流动相 ,检测波长 2 0 4nm。结果 :本方法线性范围 0 .72～ 1.6 2 μg ,回归方程Y =1.72 47× 10 -5X +1.5 34× 10 -6,r =0 .9989,平均回收率 94.45 % ,RSD为 2 .0 3% (n =6 )。结论 :本法可快速准确地测定克痨星胶囊中环黄芪醇类皂甙与黄芪甲甙的含量
Objective: To establish the quality control standard for determination of Radix Astragali in Kelixing Capsule. METHODS: The saponin from Astragalus was saponified with 1% alcoholic potassium hydroxide to convert all the acetyl groups in the sugar to hydroxyl groups. All of the saponins in the astragalus membranaceus were transformed into astragaloside by HPLC Detection. HPLC conditions: Waters NovapakC18 (3.9mm × 150mm) column, 40% methanol as the mobile phase, the detection wavelength of 204nm. Results: The linear range of the method was 0.72-1.62 μg, the regression equation was Y = 1.72 47 × 10 -5X +1.5 34 × 10 -6, r = 0.9989, the average recovery was 94.45%, RSD was 2.03 % (n = 6). Conclusion: This method can be rapid and accurate determination of kudzuoxin capsule in the content of serum saponin aloe saponin and astragaloside