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为了探讨输精管结扎术后睾丸IL—1的活性、来源及其与睾酮变化的关系,同步检测了结扎6、25个月家兔辜丸匀浆上清IL—1活性和血浆睾酮含量,并进行了睾丸组织IL—lmRNA的原位杂交.结果表明:(1)睾丸组织匀浆上清IL-1活性以VG6为高,与SOG6比较有显著差异(P<0.01);(2)睾丸组织IL-1mRNA的原位杂交,VG6中IL-lmRNA的杂交信号明显强于其他各组,杂交信号主要分布在曲细精管内的 Sertoli细胞和生殖细胞,也见于间质中的某些细胞中;(3)血浆睾酮含量结扎术后6个月明显降低,与对照组比有显著差异(P<0.05),IL-1活性与血浆睾酮含量呈明显的负相关关系(r=-0.595,P<0.01).故推测,结扎早期睾丸炎症所引起的巨噬细胞活化和Sertoli细胞吞噬变性精子和残余体过程增强,可能是睾丸IL-1的主要来源.输精管结扎术后IL-1活性的一过性升高可能是睾酮含量下降的原因.IL-1可能作为旁分泌调节因子抑制Leydig细胞睾酮合成.
To explore the relationship between the activity and source of testicular IL-1 and the changes of testosterone in the testis after vasectomy, the IL-1 activity and the plasma testosterone level in the homogenate of rabbit at 6 and 25 months after ligation were simultaneously detected The results showed that: (1) The activity of IL-1 in supernatant of testis homogenate was higher than that of SOG6 (VG6) (P <0.01); (2) The content of IL- -1 mRNA in situ hybridization, VG6 IL-lmRNA hybridization signal was significantly stronger than the other groups, the hybridization signal mainly distributed in the seminiferous tubules Sertoli cells and germ cells, also found in some of the stromal cells; ( (3) Plasma testosterone levels decreased significantly at 6 months after ligation, which was significantly lower than that of control group (P <0.05). There was a negative correlation between IL-1 activity and plasma testosterone level (r = -0.595, P <0.01 ) .Thus, it is speculated that the early testicular inflammation caused by macrophage activation and sertoli cells phagocytosis sperm and remnant enhancement process, may be the main source of testicular IL-1. After vasectomy IL-1 activity transient Increased testosterone levels may be the reason for the decline of IL-1 may act as a paracrine regulator of inhibition of Leydi g cell testosterone synthesis.