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内耳深埋于颞骨岩部,其内部和周围空隙间充满淋巴液。因此,进行内耳切片首先要克服坚硬的迷路骨性障碍和选用合适的包埋剂替代淋巴液来支持膜迷路,以免切片时破坏内耳的结构。我们在常规冰冻切片技术的基础上,应用豚鼠全内耳膜迷路分离取材术,从颞骨中将膜迷路取出,或将整个颞骨浸入盐酸溶液中脱钙的方法,来克服骨性障碍;采用低温下可以凝固的明胶溶液作为膜迷路的支持包埋剂,进行内耳冰冻切片。经4年多实践,我们认为此法具有比火棉胶制片法切片较薄、制片周期短、可以任意选择内耳终器切片角度、内耳结构保持完好等
The inner ear is deeply buried in the petrous bone and its internal and peripheral spaces are filled with lymph. Therefore, the first section of the inner ear to overcome hard and lost bony disorders and the choice of appropriate embedding agent instead of lymph to support the lost membrane, so as not to damage the structure of the inner ear during slicing. On the basis of the conventional frozen sectioning technique, we applied the whole internal eardrum isolation and extraction of guinea pigs to remove the membranous labyrinth from the temporal bone, or the whole temporal bone immersed in hydrochloric acid solution to overcome the bony disorder. Solidified gelatin solution as a labyrinth supportive embedding agent, inner ear frozen sections. After more than 4 years of practice, we believe that this method has thinner than the flagellum tablet method slice, the production cycle is short, you can choose the slice angle of the inner ear terminal, the inner ear structure remains intact, etc.