论文部分内容阅读
SRO转录因子在植物响应盐、旱和病菌等生物和非生物胁迫中具有重要的调节作用。本研究从抗枯萎病耐黄萎病的棉花品种鲁研棉32号中克隆获得2个基因:命名为GhSRO04和GhSRO08(Gen Bank登录号为KR534896和KR534895)。结果显示:GhSRO04包含一个最大开放阅读框1830 bp(ORF),推测编码609个氨基酸;GhSRO08包含一个最大开放阅读框1 842 bp(ORF),推测编码编码613个氨基酸。比对分析表明GhSRO04和GhSRO08基因均含有RCD1-SRO-TAF4结构域(RST)。系统进化树分析表明,GhSRO04和GhSRO08编码产物与拟南芥RCD1和SRO1亲缘关系最近,属于同一进化分支。实时荧光定量PCR(q RT-PCR)分析基因表达显示,GhSRO04和GhSRO08基因均受黄萎病菌侵染、干旱和高盐诱导表达,表明GhSRO04和GhSRO08在棉花应对盐、旱和病菌胁迫中可能起着重要的调节作用,对棉花种质的抗逆性遗传改良具有潜在利用价值。本研究首次从棉花中分离鉴定出SRO类转录因子,并进行了初步功能分析,为棉花遗传改良储备了基因资源。
SRO transcription factors play an important regulatory role in the response of plants to biological and abiotic stresses such as salt, drought and germs. In this study, two genes were cloned from cotton cultivar Luyan 32 which is resistant to Verticillium wilt, and named GhSRO04 and GhSRO08 (Gen Bank accession numbers KR534896 and KR534895). The results showed that GhSRO04 contained a 1830 bp (ORF) open reading frame (ORF) with a deduced 609 amino acid sequence. GhSRO08 contained a 1 842 bp open reading frame (ORF) with a putative coding of 613 amino acids. Alignment analysis showed that the GhSRO04 and GhSRO08 genes both contained the RCD1-SRO-TAF4 domain (RST). Phylogenetic tree analysis showed that the coding products of GhSRO04 and GhSRO08 had the closest genetic relationship with Arabidopsis RCD1 and SRO1 and belong to the same evolutionary branch. Analysis of gene expression by q RT-PCR showed that both GhSRO04 and GhSRO08 genes were infested by Verticillium dahliae and induced by drought and high salt, indicating that GhSRO04 and GhSRO08 may play roles in salt, drought and germ stress in cotton It plays an important regulatory role and has potential value in genetic improvement of cotton germplasm resistance. In this study, SRO transcription factors were isolated and identified from cotton for the first time, and preliminary functional analysis was carried out to reserve genetic resources for cotton genetic improvement.