论文部分内容阅读
探讨了外生菌根菌耳环乳牛肝（Ｓｕｉｌｌｕｓｇｒｅｖｉｌｌｅｉ）的原生质体制备及再生条件。用ＭＹＧ培养基培养菌丝７天、宋用１．５％Ｌｙｗａｌｌｚｙｍｅ，以０．６ｍｏｌ甘露醇为稳渗剂，在酶解液ｐＮ值５．５，温度３１℃，酶解４ｈｒ的条件下，每０．１ｇ湿菌丝的原生质体产量这６×１０￣６／ｍｌ。用ＳＯ培养基培养菌丝能提高原生质体产量，但再生率下降。综合产量和再生率考虑以ＭＹＧ培养菌丝效果较好。
The protoplast preparation and regeneration conditions of ectomycorrhizal fungi ear millet (Suillus grevillei) were discussed. Mycelium was cultured with MYG medium for 7 days, with 1.5% Lywallzyme for Song, 0.6mol mannitol as stabilizing agent, pN value of 5.5, temperature 31 ℃, hydrolysis time 4hr, Protoplast production per 0.1 g of wet mycelium this 6 × 10 ~ 6 / ml. Cultivation of mycelium with SO medium can increase protoplast production, but the regeneration rate decreased. Integrated Yield and Regeneration Considering mycelium growth in MYG is better.