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用2分辨率强度数据,以三方二锌猪胰岛素分子的原子坐标为初始模型,应用约束最小二乘方法对[Trp]~(B1)猪胰岛素结构进行了晶体学修正,R值下降到0.24。同三方二锌胰岛素相比,原来呈近似二重对称的两个独立分子的结构发生了不对称的变化。明显的构象变化产生在分子IB链N端和邻近残基A13的区域。
Using the two-resolution intensity data, the atomic coordinates of the tripartite zinc porcine insulin molecule as the initial model, the constrained least squares method was used to crystallographically modify the insulin structure of [Trp] ~ (B1) porcine insulin, and the R value decreased to 0.24. Compared with the three-party two zinc insulin, asymmetric changes have taken place in the structures of two independent molecules that were originally double-symmetric. Distinct conformational changes arise in the N-terminus of the molecular IB chain and in the region adjacent to residue A13.