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目的:完善速效牛黄丸的质量标准。方法:采用薄层色谱法对栀子、黄芩和黄连进行鉴别;采用高效液相色谱法测定盐酸小檗碱含量。结果:薄层色谱中斑点清晰,易于识别。盐酸小檗碱在0.03～0.70μg(r=0.9999)范围内与峰面积呈良好的线性关系(r=0.9999),平均加样回收率为100.6%,RSD为1.1%(n=6)。结论:该方法简单、快速、具有良好的重现性。
Objective: To improve the quality standard of quick-effect Niuhuang Wan. Methods: Gardenia jasminoides, Scutellaria baicalensis and Coptis chinensis were identified by TLC. The content of berberine hydrochloride was determined by high performance liquid chromatography. Results: The spots in TLC were clear and easy to identify. Berberine hydrochloride showed a good linear relationship with the peak area (r = 0.9999) in the range of 0.03-0.70 μg (r = 0.9999), with an average recovery of 100.6% and a RSD of 1.1% (n = 6). Conclusion: This method is simple, rapid, and has good reproducibility.