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为了探讨Parkinson病(PD)小鼠相关脑区的小胶质细胞反应性是否存在差异,本研究采用MPTP诱导的小鼠PD模型,用GSA I-B4-HRP组织化学染色方法对黑质致密部(SNc)、尾壳核(CPu)、腹侧被盖区(VTA)和额叶联合皮层(FrA)四个部位中小胶质细胞的形态和数量进行了观察;同时用免疫荧光染色检测了SNc和VTA中TH阳性神经元的数量,用Western blot方法检测CPu和FrA内TH蛋白含量的变化情况。结果显示:SNc中TH阳性神经元的数量和CPu中TH蛋白的含量在MPTP腹腔注射后1 d即大幅度降低(降幅约为68%);在MPTP腹腔注射后3 d起SNc中TH阳性神经元的数量和CPu中TH蛋白的含量逐步增加;小胶质细胞在MPTP腹腔注射后1～3 d的反应明显,然后逐渐减弱。VTA中TH阳性神经元的数量和FrA中TH蛋白的含量在MPTP腹腔注射后降幅不明显(降幅约在15%),小胶质细胞在MPTP腹腔注射后1 d的反应较为明显,其它时段的变化不显著。本研究结果提示:PD模型小鼠SNc和CPu中的小胶质细胞反应明显强于VTA和FrA区,反应性小胶质细胞在SNc中的多巴胺(DA)能神经元损伤过程中扮演了重要角色。
In order to investigate whether microglia reactivity in brain regions related to Parkinson’s disease (PD) is different, we used MPTP-induced PD model in mice and GSA I-B4-HRP histochemical staining on the substantia nigra pars compacta SNc, CPu, VTA and FrA were observed. The numbers of microglial cells in SNc, CPu, FrA were detected by immunofluorescence staining. And the number of TH-positive neurons in VTA. The content of TH protein in CPu and FrA was detected by Western blot. The results showed that the number of TH positive neurons in SNc and the TH protein content in CPu decreased significantly (by about 68%) on day 1 after MPTP injection. In the SNc of MPTP, TH positive neurons The number of metamycins and the content of TH protein in CPu gradually increased. Microglial cells responded well 1 ~ 3 days after intraperitoneal injection of MPTP, and then gradually weakened. The number of TH positive neurons in VTA and the content of TH protein in FrA were not significantly decreased (by about 15%) after intraperitoneal injection of MPTP. The response of microglia at 1 d after intraperitoneal injection of MPTP was more obvious. No significant change. Our results suggest that microglial responses in SNc and CPu in PD model mice are significantly stronger than those in VTA and FrA regions and that reactive microglia play an important role in dopamine (DA) neuronal damage in SNc Character.