论文部分内容阅读
目的观察1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的小鼠急性损伤帕金森病(PD)模型中纹状体(CPu)、黑质致密部(SNpc)、额叶皮层联合区(FrA)和腹侧被盖区(VTA)中TNF-α基因和蛋白的时程表达变化。方法用RT-PCR和Western blot方法对MPTP诱导的小鼠PD模型中相关脑区TNF-α基因和蛋白的表达量进行检测。结果MPTP诱导的小鼠PD模型中SNpc和CPu脑区TNF-α基因在给药后6h和1d有显著性表达,TNF-α蛋白在给药后12h和1d有显著性表达,其中1d均是表达高峰;VTA和FrA脑区TNF-α基因和蛋白的表达不显著。结论表达增高的TNF-α可能对SNpc和VTA脑区DA能神经元及CPu和FrA脑区的DA能神经元末梢起了损伤作用,从而为深入研究PD的治疗过程提供一定的实验依据。
Objective To observe the effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) on striatum (CPu) and substantia nigra in acute Parkinson’s disease (PD) (SNpc), frontal cortex united area (FrA) and ventral tegmental area (VTA). Methods The expression of TNF-α gene and protein in relevant brain regions of MPTP-induced mouse PD model was detected by RT-PCR and Western blot. Results TNF-α gene in SNPc and CPu brain regions of MPTP-induced mouse PD model was significantly expressed at 6h and 1d after administration, and TNF-α protein was significantly expressed at 12h and 1d after administration Expression peak; VTA and FrA brain TNF-α gene and protein expression was not significant. Conclusions Increased expression of TNF-α may damage the DA neurons in the SNpc and VTA brain regions and the DA neurons in the CPu and FrA brain regions, which may provide some experimental evidence for the further study of the PD treatment.