应用聚合酶链反应、基因克隆和末端终止技术，测定了分属ＤＲ９、ＤＲ１１和ＤＲ１２的６个中国汉族人ＨＬＡ纯合细胞ＤＰＢ１基因第二外显子的核苷酸序列。结果表明，６个纯合细胞分别带有４种等位特异性ＤＰＢ１＊０２０１２、ＤＰＢ１＊０２０２、ＤＰＢ１＊０５０１、ＤＰＢ１＊１３０１，基因结构和白种人相同，因而采用国外参考试剂和探针对中国人标本作ＤＰＢ１基因分型是可行的。同时，首次揭示了狨猴ＤＰＢ１基因第二外显子序列，该序列和人类ＤＰ基因同源性为６９．９％。 The nucleotide sequence of the second exon of DPB1 gene in HLA homozygous cells of six Chinese Han ethnic groups assigned to DR9, DR11 and DR12 was determined by polymerase chain reaction, gene cloning and terminal termination. The results showed that the six homozygous cells with four allelic-specific DPB1 * 02012, DPB1 * 0202, DPB1 * 0501, DPB1 * 1301, respectively, had the same genetic structure as Caucasians. Therefore, Chinese specimens for DPB1 genotyping is feasible. At the same time, the second exon of DPB1 gene of macaque was revealed for the first time. The sequence shared 69.9% identity with human DP gene.