论文部分内容阅读
目的:观察支气管哮喘病儿外周血单个核细胞(PBMC)来源树突状细胞(DC)功能变化。方法:以18例健康儿童为对照,选择16例支气管哮喘发作期病儿为研究对象,分离PBMC并经rhGM-CSF诱生成熟DC。采用流式细胞仪(FACS)检测DC表面共刺激分子CD80(B7-1)、CD86(B7-2)和CD83的表达率;ELISA法检测培养上清液中IL-10和IL-12的变化。结果:①哮喘组DC表面CD86的表达率明显高于健康对照组(t=2.27,P<0.05),CD80、CD83的表达率与健康对照组比较均无显著性差异(t=1.17,1.34;P>0.05)。②哮喘组DC分泌IL-10、IL-12水平均明显低于健康对照组(t’=3.31,3.39;P<0.01)。③哮喘组DC分泌IL-10与IL-12成正相关(r=0.740,P<0.01),而健康对照组IL-10与IL-12无相关性(r=0.232,P>0.05)。结论:支气管哮喘病儿DC存在功能缺陷,主要表现在CD86表达升高、IL-10、IL-12分泌减少。
Objective: To observe the changes of dendritic cells (DCs) derived from peripheral blood mononuclear cells (PBMCs) in children with bronchial asthma. Methods: Twenty-eight healthy children were selected as controls. Sixteen children with bronchial asthma were selected as experimental subjects. PBMCs were isolated and induced to mature DCs by rhGM-CSF. The expression of costimulatory molecules CD80 (B7-1), CD86 (B7-2) and CD83 on DCs were detected by flow cytometry (FCM). The changes of IL-10 and IL-12 in culture supernatants were detected by ELISA . Results: ①The expression of CD86 on DCs in asthma group was significantly higher than that in healthy controls (t = 2.27, P <0.05). The expression rates of CD80 and CD83 were not significantly different from those in healthy controls (t = 1.17 and 1.34; P> 0.05). ② The levels of IL-10 and IL-12 secreted by DCs in asthma group were significantly lower than those in healthy controls (t ’= 3.31 and 3.39; P <0.01). (3) There was a positive correlation between the secretion of IL-10 and IL-12 in asthmatic group (r = 0.740, P <0.01), but no correlation between IL-10 and IL-12 in healthy controls (r = 0.232, P> 0.05). CONCLUSION: DC in children with bronchial asthma is deficient in function, mainly manifested in increased CD86 expression and decreased secretion of IL-10 and IL-12.