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1992年,巴西Fundacao Oswaldo Cruz和Minas Gerais联邦大学及美国基因组研究所协作,从事曼氏血吸虫已表达序列标志(EST)的研究工作。起先,采用定向cDNA文库,通过对随机选择的克隆一端或两端测序而获得EST。由此获得从未报告过的曼氏
In 1992, Fundacao Oswaldo Cruz of Brazil and Minas Gerais Federal University and the United States Institute of Genomics collaborated on the research of the expressed sequence tag (EST) of Schistosoma mansoni. Initially, a directional cDNA library was used and ESTs were obtained by sequencing one or both ends of randomly selected clones. As a result, Man has never been reported