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以4个不同品种的羽衣甘蓝(Brassica oleracea var.acephala)幼苗为试材,研究了在高温胁迫8d(39±0.5)℃/(28±0.5)℃(昼/夜)后,羽衣甘蓝幼苗叶片一些抗逆境的生理生化指标及SOD、POD同工酶变化,以探讨不同羽衣甘蓝植物对高温的适应性。结果表明:随胁迫时间的延长,不同品种的羽衣甘蓝的叶绿素含量显著下降,而幼苗叶MDA、脯氨酸含量与质膜透性含量增加;在处理后2d,SOD、POD含量有较大幅度的升高,但第4天后开始出现下降,不同品种之间有显著差异;试验表明“名古屋”、“京冠红2号”比“红鸥”和“叶牡丹”有较强的耐热性;SOD、POD同工酶研究表明,“名古屋”和“叶牡丹”的SOD同工酶谱带具有相似性,而与“红鸥”与“京冠红2号”不同;“名古屋”和“京冠红2号”的POD同工酶谱带相似,而“叶牡丹”与“红鸥”的POD谱带相似,但“名古屋”和“京冠红2号”与“叶牡丹”和“红鸥”的POD谱带差异较大。
Four different varieties of Brassica oleracea var. Acephala seedlings were used as experimental materials to study the effects of high temperature stress on the leaves of kale seedlings after 8 days (39 ± 0.5) ℃ / (28 ± 0.5) ℃ (day / night) Physiological and biochemical indexes of some anti-stress and changes of SOD and POD isoenzymes were investigated to investigate the adaptability of different Kale plants to high temperature. The results showed that the chlorophyll content of Kale of different varieties decreased significantly with the prolongation of stress time, while the content of MDA, proline and membrane permeability of seedling leaves increased with the prolongation of stress time. On the 2nd day after treatment, the contents of SOD and POD were more significant The results showed that “Nagoya”, “Jinguanhong 2”, “Hongwu” and “Peony” “SOD and POD isoenzymes showed that SOD isozyme bands of” Nagoya “and” Ye Peony “had similarities with” Red Gull “and” “” Jinghong red 2 “;” Nagoya “and” Jinguanhong 2 “POD isozyme bands are similar, and” leaf peony “and” red gull “ POD bands were similar, but the POD bands of ”Nagoya“ and ”Jinguanhong 2“ and ”Ye Peony“ and ”Red Gull" were quite different.