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目的研究强直性脊柱炎患者血清中抗DNA酶B测定的临床价值。方法采用胶乳凝集法和酶联免疫吸附法(ELISA)测定2009年4—9月山东大学附属省立医院风湿免疫科门诊和病房收治的65例强直性脊柱炎(AS)患者和96名正常人血清中抗溶血性链球菌溶血素O(ASO)和抗DNA酶B抗体滴度,并按抗DNA酶B滴度阳性和阴性分成两组进行临床分析。结果 AS组ASO阳性率为47.7%,显著高于对照组(20.8%,P<0.05);AS组DNA酶B抗体阳性率58.5%,显著高于对照组(25.0%,P<0.05)。DNA酶B抗体和ASO两种抗体的检测在AS患者中差异无统计学意义(P>0.05)。抗DNA酶B阳性组的AS患者平均年龄明显低于阴性组(P<0.05)。AS患者抗DNA酶B阳性组膝关节、踝关节肿痛、臀部疼痛、“4”字征阳性、WBC升高、C反应蛋白升高的发生率明显高于阴性组(P<0.05)。结论 AS患者溶血性链球菌感染发生率高于正常人,链球菌感染与AS病情活动有一定的关系。
Objective To study the clinical value of serum anti-DNase B assay in patients with ankylosing spondylitis. Methods Sixty-five patients with ankylosing spondylitis (AS) and 96 normal subjects (admitted to the Department of Rheumatology, Shandong Provincial Affiliated Provincial Hospital from April to September 2009) were enrolled in this study by latex agglutination and enzyme-linked immunosorbent assay (ELISA) Serum anti-hemolytic streptolysin O (ASO) and anti-DNase B antibody titers, and anti-DNase B titer positive and negative were divided into two groups for clinical analysis. Results The ASO positive rate in AS group was 47.7%, which was significantly higher than that in control group (20.8%, P <0.05). The positive rate of DNase B antibody in AS group was significantly higher than that in control group (25.0%, P <0.05). The detection of DNase B antibody and ASO antibody in AS patients showed no significant difference (P> 0.05). The average age of anti-DNase B-positive AS patients was significantly lower than that of negative patients (P <0.05). In AS patients, the incidence of knee joint, ankle joint pain, gluteal pain, positive signs of “4”, elevated WBC and elevated C-reactive protein in AS patients were significantly higher than those in the negative group (P <0.05) . Conclusions The incidence of hemolytic streptococcus infection in AS patients is higher than that in normal people, and the relationship between streptococcal infection and AS activity has a certain relationship.