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对20例慢性丙型肝炎(CHC)患者应用干扰素(IFN)治疗前后血清 HCV RNA 进行定量检测,探讨血清 HCV RNA 量及其量的变化与 IFN 疗效的关系。材料与方法一、临床资料20例 CHC 患者,男14例,女6例,平均年龄41.2±10.3岁,诊断符合1995年5月北京第五次全国传染病寄生虫病学术会议讨论修订的病毒性肝炎防治方案。所有病例均接受 IFN-α2b治疗,3MU 肌肉注射,每周3次,疗程6个月。血清标本分别来自 IFN 治疗前、治疗结束和停药后半年。二、方法1.血清 HCV RNA 定量方法:利用信号引物能量转移技术,采用美国 AG-9600型定量 PER 扩增仪进行检测。2.HCV RNA 定量判定标准:每次实验各设阳性对照、阴性对照、空白对照和最大信号对照,每份标本取两孔均值计算
Serum HCV RNA was detected in 20 patients with chronic hepatitis C (CHC) before and after treatment with interferon (IFN) to explore the relationship between the change of serum HCV RNA quantity and IFN efficacy. Materials and Methods 1. Clinical data 20 CHC patients, 14 males and 6 females, with an average age of 41.2 ± 10.3 years, diagnosed in line with the Fifth National Conference on Infectious Diseases Parasitology in Beijing in May 1995 to discuss the revision of the virus Hepatitis control program. All cases were treated with IFN-α2b, 3MU intramuscular injection, 3 times a week for 6 months. Serum samples were from pre-IFN treatment, treatment and drug withdrawal after six months. Second, methods 1. Serum HCV RNA quantitative methods: the use of signal primer energy transfer technology, using the United States AG-9600-based quantitative PER amplification for testing. 2. HCV RNA quantitative criteria: each experiment positive control, negative control, blank control and maximum signal control, each sample taken two-hole average