对植物组织DNA抽提是在分子水平上对植物进行系统分析与研究的基础性工作.本文提供了一种从小麦干种子中快速提取总DNA的优化方法,该方法不需液氮冷冻或是冻干组织脱水和水浴,用氯仿对材料进行预处理,使用1.5%的SDS抽提缓冲液和氯仿/异戊醇处理,并用预冷的无水乙醇沉淀DNA,即可在短时间内得到小麦基因组总DNA.实验证明,用该方法每300mg干种子可得到约30-40μg的总DNA,其产量和质量均适合进行SSR分析. DNA extraction from plant tissues is the basic work to systematically analyze and study plants at the molecular level.This paper provides an optimized method for rapid total DNA extraction from dried wheat seeds which does not require liquid nitrogen freezing or The lyophilized tissue is dehydrated and waterbath, the material is pretreated with chloroform, treated with 1.5% SDS extraction buffer and chloroform / isoamyl alcohol, and precipitated with precooled absolute ethanol to obtain wheat in a short period of time Total genomic DNA. Experiments show that using this method for every 300mg dry seeds can get about 30-40μg of total DNA, its yield and quality are suitable for SSR analysis.