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目的 :观察碱性成纤维细胞生长因子 (bFGF)在低氧性肺动脉高压发病中的可能作用。方法 :应用逆转录多聚酶联反应 (RT -PCR)技术观察低氧大鼠肺动脉bFGF的表达水平 ,并观察bFGF对离体大鼠肺动脉环收缩功能的影响。结果 :①低氧时 ,肺动脉bFGFmRNA表达明显增加 ,bFGF与 β -actin的RT -PCR反应产物的放射强度 (counts·min-1)之比由对照的 0 5 0 74上升到 0 9182 (低氧 1周 )和 0 7812 (低氧 2周 ) (P均 <0 0 5 )。②bFGF能收缩离体大鼠肺血管环 ,收缩强度与剂量相关 (r=0 6 95 ,P <0 0 5 ) ,其EC50 为 2 6 2× 10 -7mol/L。结论 :提示bFGF可能参与低氧性肺动脉高压的发病过程。
Objective: To investigate the possible role of basic fibroblast growth factor (bFGF) in the pathogenesis of hypoxic pulmonary hypertension. Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to observe the expression of bFGF in pulmonary artery of hypoxic rats. The effect of bFGF on pulmonary artery systolic function was observed. RESULTS: ① In hypoxia, the expression of bFGF mRNA in pulmonary artery increased significantly, and the ratio of counts · min-1 of RT-PCR products of bFGF and β-actin rose from 0 5074 to 0 9182 (hypoxia 1 week) and 0 7812 (hypoxia 2 weeks) (all P <0 05). BFGF can reduce the pulmonary vascular ring in isolated rats, and the contractile intensity was dose-dependent (r = 0 695, P <0 05). The EC50 was 226 × 10 -7 mol / L. Conclusion: It suggests that bFGF may be involved in the pathogenesis of hypoxic pulmonary hypertension.