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本研究目的是构建既可鉴定花生种质资源耐低温属性,又可进行耐低温性数量性状分析的表型鉴定方法。本方法构建公式相对发芽率=x_i/y_i×100%,花生耐低温性属性分级标准鉴定花生耐低温属性,及利用相对发芽率数据进行耐低温数量性状分析。利用此方法对94份花生种质资源进行鉴定,鉴定出相对发芽率≥85%耐低温型(R)6个,约占检测资源的6.38%,分别为山花8(A1)、豫花1(A38)、开农30(A10)、白沙1016(A37)、油4(A32)和泉花646(A42),从94份花生资源中选取20份材料进行田间耐低温验证,其结果与本研究构建的方法结果一致,证明花生种质资源耐低温表型方法可以鉴定花生种质资源耐低温属性;利用此方法鉴定出徐花13×中花6重组自交系群体(RIL)各家系及亲本,分析耐低温性主基因+多基因遗传模型,符合模型G-1,主基因的遗传率为91.21%,多基因的遗传率为8.34%,证明此方法可应用在耐低温性数量性状分析上。
The purpose of this study is to construct phenotypic identification methods that can identify the low temperature resistance of peanut germplasm resources and the quantitative trait analysis of low temperature resistance. The relative germination rate of this method was x_i / y_i × 100%. The peanut low temperature tolerance classification was used to characterize the peanut low temperature tolerance and the relative germination rate was used to analyze the quantitative characters of low temperature tolerance. 94 peanut germplasm resources were identified by this method. Six germplasm resources with relative germination rate ≥85% and R% 6 (accounting for 6.38% of the total) were identified as Shanhua 8 (A1), Yuhua 1 ( A38, A10, Baisha 1016 (A37), oil 4 (A32) and Quanhua 646 (A42), 20 materials were selected from 94 peanut resources for field low temperature verification. The results were in agreement with the results of this study The results showed that the low temperature resistance phenotype of peanut germplasm resources could be used to identify the low temperature tolerance of peanut germplasm resources. By using this method, we identified each line and parent of RIL population of Xuhua 13 × Zhonghua 6 , The analysis of low temperature resistant major gene + polygene genetic model, in line with the model G-1, the heritability of the major gene was 91.21%, the heritability of multiple genes was 8.34%, proves that this method can be applied in quantitative character analysis of low temperature tolerance .