论文部分内容阅读
目的利用响应面法确定蘘荷总黄酮的最佳超声辅助提取工艺,并探讨其抗氧化活性。方法在单因素实验的基础上,进行4因素3水平的Box-Behnken中心组合实验设计,利用响应面分析优化工艺条件,并对此工艺下的提取液进行抗氧化活性检测。结果在超声功率300 W时,蘘荷总黄酮最佳提取工艺条件为处理温度60℃、乙醇体积分数85.99%、液料比98.08 mL·g~(-1)、处理时间15 min,此工艺条件下蘘荷总黄酮得率为37.01%,与预测值35.49%的相对误差仅4.28%;1.038 7mg·mL~(-1)囊荷总黄酮与56.60μg·mL~(-1)Vc的还原力相当,一定浓度的囊荷总黄酮对·OH、DPPH自由基清除率达到73.07%、65.72%,对脂质体过氧化的抑制率达到49.94%。结论蘘荷总黄酮可作为一种新型抗氧化药物开发,其提取工艺可用响应面法进行回归分析和参数优化。
Objective To determine the optimal ultrasonic-assisted extraction of total flavonoids from Rhizoma Polygoni cuspidati by response surface methodology and to investigate its antioxidant activity. Methods Based on the single factor experiment, the Box-Behnken central design experiment with 4 factors and 3 levels was designed. The response surface analysis was used to optimize the process conditions and the antioxidant activity of the extracts was tested. Results The optimal extraction conditions of total flavonoids were as follows: temperature 60 ℃, ethanol volume fraction 85.99%, liquid ratio 98.08 mL · g -1, processing time 15 min at ultrasonic power 300 W, The total yield of total flavonoids was 37.01%, with a relative error of 4.28% from the predicted value of 35.49%. The reducing power of 1.038 7 mg · mL -1 total flavonoids and 56.60 μg · mL -1 Vc The results showed that the free radical scavenging rate of · OH and DPPH reached 73.07% and 65.72% at a certain concentration, and the inhibition rate of liposome peroxidation reached 49.94%. Conclusion The total flavonoids from Rhizoma Chuanxiong could be developed as a new type of anti-oxidant drugs. The extraction process can be used for regression analysis and parameter optimization by response surface methodology.