论文部分内容阅读
目的:研究维生素K2(VK2)对人骨髓增生异常综合征细胞株MUTZ-1的生长抑制作用。方法:采用光镜或电镜技术观察VK2作用于MUTZ-1细胞株后细胞形态和超微结构的改变;应用四甲基偶氮唑蓝(MTT)法检测细胞的增殖抑制作用。结果:VK2作用于MUTZ-1 72 h后呈现典型凋亡细胞的形态学改变,且随着VK2浓度的增加和作用时间的延长凋亡细胞所占的百分比增高;MTT结果显示10μmol.L-1及以上浓度的VK2处理MUTZ-1细胞株后细胞生长明显受抑,并呈浓度依赖性,与对照组比有显著性差异(P<0.05);且随着VK2作用时间的延长细胞凋亡率也逐渐增高,且呈明显的时间依赖性(P<0.05)。5μmol.L-1的VK2有促MUTZ-1细胞株增生的趋势,但与对照组相比无显著性差异(P>0.05)。结论:低浓度VK2(5μmol.L-1)对MUTZ-1细胞株有促增殖趋势;较高浓度的VK2(10~40μmol.L-1)主要是通过诱导MUTZ-1细胞凋亡而抑制其增殖。
Objective: To study the inhibitory effect of vitamin K2 (VK2) on the growth of human myelodysplastic syndrome cell line MUTZ-1. Methods: The morphological and ultrastructural changes of VK2 cells treated with VK2 were observed by light microscopy or electron microscopy. The cell proliferation was evaluated by MTT assay. RESULTS: The morphological changes of typical apoptotic cells were observed after 72 h treatment of VK2, and the percentage of apoptotic cells increased with the increase of VK2 concentration and the prolongation of action time. The MTT assay showed that 10 μmol·L-1 (P <0.05). With the prolongation of VK2 treatment time, the apoptosis rate of MUTZ-1 cells was significantly inhibited (P <0.05) Also gradually increased, and showed a significant time-dependent (P <0.05). VK2 with 5μmol.L-1 had the tendency of promoting the proliferation of MUTZ-1 cell line, but no significant difference compared with the control group (P> 0.05). CONCLUSION: VK2 (5μmol.L-1) can promote the proliferation of MUTZ-1 cell line. VK2 (10-40μmol.L-1) mainly inhibits MUTZ-1 cell apoptosis by inducing apoptosis proliferation.