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在离体条件下EB病毒能转化人脐带血淋巴细胞,虽然建株的淋巴细胞(LcL)带有病毒基因组并持续表达EB病毒核抗原(EBNA)但通常不产生感染性病毒,从而认为可能存在着宿主细胞对病毒的控制作用。本作者和其他的报道指出经致死照射的建株细胞和原代脐带血淋巴细胞或棉顶猴外周血淋巴细胞共同培养能诱导病毒产生。这些结果指出大多数细胞株均产生微量的感染性EB病毒。新鲜肝素处理的人脐带血经离心得到白细胞,悬浮在20%胎牛血清的完全培养液中,细胞密度为2×10~6/ml,二甲亚砜(DMSO)的浓度为1～2%。从B95—8细
In vitro, Epstein-Barr virus (EBV) can transform human umbilical cord blood cells. Although established lymphocytes (LcL) harbor the viral genome and continue to express Epstein-Barr virus nuclear antigen (EBNA) but do not usually produce infectious virus, they may be considered The host cell on the virus control. This and other reports indicate that co-culture of lethally irradiated establishment cells with primary cord blood lymphocytes or cotton monkey peripheral blood lymphocytes can induce virus production. These results indicate that most cell lines produce minimal amounts of infectious Epstein-Barr virus. Fresh heparin-treated human umbilical cord blood was centrifuged to obtain leukocytes, suspended in 20% fetal bovine serum in complete medium, a cell density of 2 × 10 ~ 6 / ml, dimethyl sulfoxide (DMSO) concentration of 1 to 2% . Fine from B95-8