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气液色谱法(GLC)能鉴别萄葡糖无氧酵解时所产生的挥发性脂肪酸(VFA),从而可证实厌氧菌的存在。作者对9名正常人的唾液和83例胸膜肺疾患病例的粘液脓痰或胸腔脓液作了厌氧培养和GLC检查,旨在确定不同疾病组厌氧菌感染的发生率,评价GLC在有临床意义的厌氧菌感染诊断中的价值。GLC是将气液两相间某一混合物各组分开的一种分离方法。惰性气体(例如氮气)作为移动相,涂有液体材料的惰性固体柱作为固定相。气化的标本在色谱柱入口进入载气流。当惰性气体不断通过色谱柱时,标本的各组分被分配到两相之间。由于亲合力的不同,标本各组分在色谱柱出口出现了分离带,再通过检测和记录,就可以见到含有色谱蜂的色谱图。9名健康人唾液常规厌氧培养均有类杆菌生长。
Gas liquid chromatography (GLC) identifies the presence of anaerobic bacteria by identifying volatile fatty acids (VFA) produced by anaerobic glycolysis of glucose. The authors conducted anaerobic culture and GLC examinations of mucous purulent sputum or pleural pus in 9 normal saliva and 83 pleural lung disease cases to determine the prevalence of anaerobic infections in different disease groups and to evaluate the effect of GLC on Clinical significance of anaerobic infections in the diagnosis. GLC is a separation method that separates each group of a mixture of gas and liquid phases. An inert gas (eg nitrogen) was used as the mobile phase, and a column of inert solid coated with a liquid material was used as the stationary phase. Gasified specimens enter the carrier gas stream at the column inlet. As inert gas continuously passes through the column, the components of the specimen are dispensed between the two phases. Due to the different affinities, the sample components appeared in the column outlet separation band, and then through the detection and recording, you can see the chromatogram containing the chromatogram bees. 9 normal human saliva bacteria are anaerobic culture are Bacteroides growth.